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. 1999 Jun 7;189(11):1747–1756. doi: 10.1084/jem.189.11.1747

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Expression of BAFF. (A) Northern blots (2 μg poly A⁺ RNA per lane) of various human tissues were probed with BAFF antisense mRNA. (B) Reverse transcriptase amplification of BAFF, IL-2 receptor α chain (IL2-Rα), and actin from RNA of purified blood T cells at various time points of PHA activation, E-rosetting–negative blood cells (mostly B cells), in vitro–derived immature dendritic cells, 293 cells, and 293 cells stably transfected with full-length BAFF (293-BAFF). Control amplifications were performed in the absence of added cDNA. IL-2 receptor α chain was amplified as a marker of T cell activation.