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. 1999 Jun 7;189(11):1747–1756. doi: 10.1084/jem.189.11.1747

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BAFF binds to mature B cells. (A) Binding of sBAFF to BJAB and Jurkat cell lines, and to purified CD19⁺ cells of cord blood. Cells were stained with the indicated amount (in ng/50 μl) of Flag-BAFF and analyzed by flow cytometry. (B) Binding of sBAFF to PBLs. PBLs were stained with anti-CD8–FITC or with anti-CD19–FITC (x axis) and with Flag-BAFF plus M2-biotin and avidin-PE (y axis). Flag-BAFF was omitted in controls.