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. 1999 Jun 7;189(11):1839–1845. doi: 10.1084/jem.189.11.1839

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Defective IKK and NF-κB activation in IKKβ-deficient EF cells. Second passage EFs from E11.5 Ikkβ^+/+, Ikkβ^+/−, and Ikkβ^−/− embryos were stimulated with TNF-α or IL-1. At the indicated times, whole cell extracts were prepared and used to measure (A) IKK activity (KA), and (B) NF-κB DNA binding activity. IB, immunoblotting.

Defective IKK and NF-κB activation in IKKβ-deficient EF cells. Second passage EFs from E11.5 Ikkβ^+/+, Ikkβ^+/−, and Ikkβ^−/− embryos were stimulated with TNF-α or IL-1. At the indicated times, whole cell extracts were prepared and used to measure (A) IKK activity (KA), and (B) NF-κB DNA binding activity. IB, immunoblotting.