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. 1999 Jun 7;189(11):1847–1852. doi: 10.1084/jem.189.11.1847

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(A) RNA blot of SGD patient bone marrow total RNA and normal human bone marrow pA-RNA. Three concentrations of control RNA, 1, 0.5, and 0.25 μg, and 10 μg of SGD patient RNA were used, as indicated below the lanes. Blot was probed with [³²P]dCTP-labeled probes, as indicated, and stripped between hybridizations. (B) Immunoblotting of cellular proteins extracted from peripheral blood neutrophils isolated as described. Top panel was blotted with rabbit anti–human polyclonal C/EBPε antibody. Center panel was blotted with rabbit anti–rat C/EBP–related protein 1 (Santa Cruz Biotechnology). Bottom panel shows immunoblotting with mouse anti–human actin antibody. Arrowheads indicate C/EBPε³², C/EBPε²⁷, and C/EBPε¹⁴ isoforms.

(A) RNA blot of SGD patient bone marrow total RNA and normal human bone marrow pA-RNA. Three concentrations of control RNA, 1, 0.5, and 0.25 μg, and 10 μg of SGD patient RNA were used, as indicated below the lanes. Blot was probed with [³²P]dCTP-labeled probes, as indicated, and stripped between hybridizations. (B) Immunoblotting of cellular proteins extracted from peripheral blood neutrophils isolated as described. Top panel was blotted with rabbit anti–human polyclonal C/EBPε antibody. Center panel was blotted with rabbit anti–rat C/EBP–related protein 1 (Santa Cruz Biotechnology). Bottom panel shows immunoblotting with mouse anti–human actin antibody. Arrowheads indicate C/EBPε³², C/EBPε²⁷, and C/EBPε¹⁴ isoforms.