Table 1.
Specificity of Immunopurified Abs against MSP3 and RESA Peptides Detected by IFA and ELISA
| IFA | ELISA | |||
|---|---|---|---|---|
| Abs | Schizonts | Rings | MSP3 | RESA |
| N-IgG | (−) | (−) | 58 | 62 |
| HI-IgG | 1/12,000 | 1/12,000 | 908 (16) | 859 (14) |
| MSP3b-IgG | 1/400 | (−) | 942 (16) | 39 (0.6) |
| RESA-IgG | (−) | 1/1,200 | 37 (0.6) | 821 (13) |
IFA was performed on slides containing a high percentage of ring forms for anti–RESA-IgG and a high percentage of schizonts for anti–MSP3b-IgG. The ratio (in parentheses) was calculated by dividing the optical density of each IgG preparation by the optical density of normal human IgG (N-IgG) against each peptide.