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. 2000 Dec 4;192(11):1535–1544. doi: 10.1084/jem.192.11.1535

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Induction of melanoma-specific CTLs by killed melanoma cell–loaded DCs. Purified CD8⁺ T cells are cultured for 3 wk with unloaded (UL-DC) or Me275-loaded DCs (L-DC). (a) IFN-γ ELISPOT using as targets DCs, either unpulsed or pulsed with the four melanoma peptides (4P). Representative experiment from three performed using DCs and T cells from different donors. (b) CTL activity in a 4-h chromium-release assay using unpulsed and melanoma peptide–pulsed (4P; mean values of three experiments) and control peptide PSA-pulsed T2 cells (one experiment).