Figure 5.
DCs loaded with killed melanoma cells prime naive T cells. Naive CD8+CD45RA+CD45RO− T cells are cultured for 3 wk with unloaded DCs (UL-DC) or with DCs loaded with killed melanoma cells (L-DC). T cells are tested in a 4-h chromium-release assay. (a) CTLs elicited by DCs loaded with Me275 bodies kill the T2 cells pulsed with different melanoma peptides (paired two-tailed t test on log transformed data comparing the killing of unpulsed and peptide-pulsed T2 cells). (b) Naive CD27+ CD45RA+CD8+ T cells differentiate into CTLs able to kill the cell line used for immunization, and (c) T2 cells pulsed with melanoma peptides (4P) but not PSA peptide (representative of two experiments).