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. 2000 Dec 4;192(11):1535–1544. doi: 10.1084/jem.192.11.1535

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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DCs loaded with killed melanoma cells stimulate melanoma-specific CD8⁺ T cells from the blood of patients with metastatic melanoma. Purified CD8⁺ T cells are cultured for 3 wk with unloaded (UL-DC) or Me275-loaded DCs (L-DC). (a) IFN-γ ELISPOT using DCs, either unpulsed or pulsed with four melanoma peptides (4P). Results from one patient. (b) T cells are able to kill Me275 used for immunization, and the killing is increased when Me275 cells are pulsed with the four melanoma peptides. Similar results were obtained in two patients. (c) CD8⁺CD45RA⁺CD45RO⁻ T cells cultured for 3 wk with DCs loaded with killed Me275 cells, kill T2 cells pulsed with four melanoma peptides.