Figure 3.
The adherence and invasion of the strain F62 and its isogenic derivatives to ME180 cells. ME180 cells (2 × 105 cells/well) were incubated with 106 CFU gonococci at 37°C for 6 h. The cells were treated or not treated with gentamicin for 2 h at 37°C. The cells were washed and lysed. Appropriate dilutions of the cell lysates were plated onto GCK agar plates. The gentamicin-resistant gonococci were counted as the internalized bacteria, and plotted as the number of gonococci (GC) per thousand cells (A). The numbers of viable gonococci from the cells untreated with gentamicin were counted as the total number of cell-associated gonococci, and plotted as the number of gonococci per cell (B). The results are the average of five independent experiments.