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. 2000 Mar 20;191(6):977–984. doi: 10.1084/jem.191.6.977

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Binding of Stat5 to the bcl-x promoter in blast crisis and chronic phase CML cells. CD34⁺ cells were isolated from the peripheral blood of patients with CML in blast crisis and chronic phase, and treated for 3 h with CGP 57148 or tyrphostin AG 555. An EMSA was performed using a radiolabeled bcl-x probe. Nuclear extracts from untreated cells (C) were preincubated with antibodies specific for Stat5 (+anti-ST5), and with an excess of an unlabeled bcl-x probe as a specific competitor (+SP) or with an irrelevant nonspecific probe (+non-SP).