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. 2000 Mar 20;191(6):977–984. doi: 10.1084/jem.191.6.977

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Analysis of Bcl-x_L and cell viability in chronic phase and blast crisis CML cells. CD34⁺ cells were isolated from the peripheral blood of patients with CML in chronic phase or in blast crisis and treated with tyrphostin AG 555 or CGP 57148 in the presence or absence of IL-3. Normal CD34⁺ cells were treated with the same inhibitors in the presence of IL-3. (A) At the indicated times, cells were analyzed for the expression of Bcl-x_L by Western blot. The levels of β-tubulin were also analyzed to assure equal loading. C, Untreated control cells. (B) Viability of normal and CML cells was measured by trypan blue dye exclusion at different times of incubation with CGP 57148. All data points represent the mean of triplicate cultures ± SD.