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. 2000 Mar 20;191(6):927–936. doi: 10.1084/jem.191.6.927

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Antigen presenting activity of DCs in vivo after culture with graded doses of HEL, minus (open symbols) or plus (closed symbols) CD40L as a maturation stimulus. As described in the legend to Fig. 5, graded doses of antigen (top) were used to pulse C3H/He DCs overnight. The washed, unfixed cells were injected subcutaneously into syngeneic mice, 2 × 10⁵ per paw. 5 d later, cultured draining lymph node cells were challenged with different doses of HEL protein (x-axis), and ³H-TdR uptake measured at 42–48 h (y-axis). Data are shown as stimulation relative to the background cpm in lymph nodes cultured without antigen. One of three similar experiments.