Figure 2.
Reciprocal effects of IL-6– and IL-2–related cytokines on resting and activated T cells. LN T cells were isolated from untreated C57BL/10 mice (A) or from C57BL/10 mice 2 d after intravenous injection with 150 mg SEB (B). T cells were plated with the indicated amounts of cytokines at a density of 2 × 105 cells per well in a 96-well plate. After 36 h in culture, the cells were stained for Vβ8.1,2 and analyzed by PI exclusion for cell death as described in Materials and Methods. Cells were plated in duplicate wells and the mean of each duplicate is indicated. This figure shows one of three experiments with similar results.