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. 2000 Mar 20;191(6):915–926. doi: 10.1084/jem.191.6.915

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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IL-6 induced phosphorylation of Stat3 at Tyr₇₀₅ in both activated and resting T cells. T cells from C57BL/10 LNs were sorted into resting (Vβ4,6,11,14) and activated (Vβ8) as described in Materials and Methods. Cells were treated with 10 ng/ml IL-6 or medium alone for 20 min and lysed. Each lane contains 2.5 × 10⁶ cell equivalents. The top panels show the anti–phospho-Stat3 (Tyr₇₀₅) blot (P-Tyr Stat3). The bottom panels show the total Stat3 in each lane after stripping and reprobing. This is one of three experiments with similar results.