Table 2.
Mean Fluorescence of HEL-binding Receptor Levels in IgM/G Pro-B Cells and IgG1 Pro-B Cells
| Tg line | Tg copy no. | Analysis 1 | Analysis 2 | Analysis 3 | Analysis 4 | Analysis 5 | Analysis 6 | Mean ± SD |
|---|---|---|---|---|---|---|---|---|
| IgM | 2 | 0.15 | 0.15 | 0.15 | 0.15 | – | 2.0 | 0.5 ± 0.8 |
| IgM/G μγ2 | 3 | 1.8 | 2.0 | 3.0 | 1.5 | 4.0 | 3.5 | 2.6 ± 1.0 |
| IgM/G μγ1 | 8 | 8.0 | 6.0 | 8.0 | 7.0 | 15.0 | 15.0 | 10.4 ± 4.3 |
| IgM/G μγ8 | 15 | – | 15.0 | – | 25.0 | 25.0 | 30.0 | 23.8 ± 6.3 |
| IgM | 2 | 0.15 | 0.15 | – | – | – | – | 0.15 ± 0.8 |
| IgG1 γγ4 | 2 | 0.5 | 0.6, 0.5, 0.5 | – | – | – | – | 0.5 ± 0.1 |
| IgG1 γγ1 | 6 | 4.0 | 3.5, 3.0, 1.5 | – | – | – | – | 3.0 ± 1.1 |
Bone marrow cells were stained as in Fig. 5. Mean fluorescence levels of HEL-binding receptors on pro-B1 (B220+S7+HSAlow) cells from six (IgM/G) and two (IgG1) separate FACS® analyses were tabulated.