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. 2000 Mar 20;191(6):937–948. doi: 10.1084/jem.191.6.937

Figure 4.

Figure 4

Analysis of cytotoxicity mechanisms used by CD1c-reactive and prenyl pyrophosphate–specific γ/δ T cells. CD1c-reactive Vγ2/Vδ1 T cells, JR.2 and IDP2, and prenyl pyrophosphate antigen–specific Vγ2/Vδ2 T cell clones, 12G12, HD.108, CP.1.15, and DG.SF68, were used in cytolytic assays performed in the presence or absence of anti-Fas mAb to block Fas-mediated cytolysis or after treatment with strontium ions to block perforin-mediated cytolysis. The mycolic acid–specific, CD1b-restricted, CD4−CD8− α/β T cell line, DN1, is shown as a control. MEP is an alkyl phosphate analogue of the IPP antigen recognized by Vγ2/Vδ2 T cells and was used to stimulate the prenyl pyrophosphate–specific clones. The E/T ratio was 10:1. Targets for CD1c-reactive and prenyl pyrophosphate–specific γ/δ T cells were C1R CD1c cells. Targets for the CD1b-restricted clone were C1R CD1b cells that had been incubated for 16 h at 37°C with M. tuberculosis sonicate at 1 μg/ml. Note that the cytolytic activity mediated by γ/δ T cells was blocked by both treatments and thus depends on both granule secretion and Fas–FasL interactions.