Table 1.
Percent Contributions of CD4 and HV4 Regions to Binding SIYR/Kb and QL9/Ld
| SIYR/Kb | QL9/Ld | |
|---|---|---|
| CDR1α | 26 | 33 |
| CDR2α | 17 | 17 |
| HV4α | −3 | 2 |
| CDR3α | 7 | 10 |
| CDR1β | 18 | 15 |
| CDR2β | 15 | 14 |
| HD4β | 11 | 0 |
| CDR3β | 9 | 9 |
| Vα | 47 | 62 |
| Vβ | 53 | 38 |
The percentage of total energy distributed among the different CDR and HV regions was calculated from log reactivity values (SIYR/Kb) or free energy (ΔΔG) values (QL9/Ld from reference 17). To standardize the comparison, maximum reductions in binding for each assay were set at 15-fold (because of the slightly different sensitivities of the two assay formats). Residues that were not tested for QL9/Ld binding (see text) were not included in the SIYR/Kb calculations.