Table 2.
MK16 Reactivity with CNS Tissue from 11 Different MS Cases
| DR2 positive | ||||
|---|---|---|---|---|
| Patient no. | DRB1 alleles | Microglia/macrophage | Astrocytes | Other |
| F31 | 01, 1501 | + | + | blood vessels |
| F37 | 03, 1501 | +/− | monocytes | |
| F32 | 1501, 1601 | +/− | ||
| F38 | 03, 1501 | +++ | +/− | |
| F55 | 1501 | + | +/− | |
| F36 | 08, 1501 | ++ | ||
| F27 | 1501 | ++ | ||
| F56 | 1501 | + | ||
| M69 | 11, 1502 | +/− | monocytes | |
| DR2 negative | ||||
| M46 | 03, 13 | blood vessels | ||
| F56 | 11, 13 | blood vessels | ||
Frozen sections of CNS tissue from 11 MS cases were HLA typed and, after fixation, stained with the following antibodies: anti–HLA-DR-DP-DQ or MK16 and anti-CD14 (microglia of the monocyte lineage, monocytes and macrophages) or CD68 (microglia and macrophages) or anti-GFAP (astrocytes) antibodies. Slides were scored in a blinded fashion by two observers on a scale from 0 to 4 based on the degree of positivity.