Table 2.
Recovery of p273 from Transfected DNA by Bacterial Transformation
| Bacterial Ampr transformants | ||||||||
|---|---|---|---|---|---|---|---|---|
| Cell line | Endogenous SR activity | n | Total | EcoRIr | % EcoRIr | EcoRIr-S–S | % S–S Rec | P value |
| A20 | − | 4 | 23,920 | 16 | 0.067 | 0 | <0.004 | NS |
| EL4 | − | 4 | 31,074 | 18 | 0.058 | 0 | <0.003 | NS |
| M12 | − | 5 | 51,456 | 7 | 0.014 | 0 | <0.002 | NS |
| CH12.LX | + | 4 | 13,898 | 16 | 0.115 | 10 | 0.072 | |
| 1B4.B6.10 | + | 3 | 9,584 | 13 | 0.136 | 4 | 0.041 | <0.001 |
| I.29μ | + | 4 | 20,968 | 39 | 0.186 | 28 | 0.133 | <0.001 |
| B cells | + | 2 | 8,340 | 21 | 0.252 | 12 | 0.144 | <0.001 |
Plasmid was transfected into the indicated cell lines. The DNA recovered from nuclei of p273-transfected cells was either left untreated or digested with EcoRI and then transformed into bacteria. The EcoRIr colonies were prepared as minipreps and analyzed by restriction mapping to identify S–S recombinant plasmids.