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. 2000 Apr 17;191(8):1319–1332. doi: 10.1084/jem.191.8.1319

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Amino acid sequence of Bam32 and localization of the Bam32 gene to human chromosome 4q25–q27. In a screen for genes selectively expressed in human GC cells, we identified Bam32, a novel signaling adaptor protein containing an SH2 and a PH domain (see Materials and Methods). (A) Amino acid sequence of human and mouse Bam32, with the SH2 domain indicated by black underline and the PH domain indicated by gray underline. The putative tyrosine-phosphorylation site is boxed. We have sequenced three cDNA clones and a RACE product corresponding to the hbam1 splice form. The COOH-terminal sequence of the hbam2 splice form, which corresponds to our mouse Bam32 clone, is derived from expressed sequence tag sequences (accession nos. AA459342 and AF150266). The Bam32 cDNA sequence is available from EMBL/GenBank/DDBJ under accession nos. AF186022 (human) and AF186023 (mouse). (B) FISH analysis showing the chromosomal location of the human Bam32 gene.