Figure 3.

Flow cytometry plots illustrate the selectivity of chemokine responses by antigenically defined B cell subsets. Unfractioned BM cells were prepared and added to inserts that were placed in wells containing either 100 nM SLC (left) or 500 nM BCA-1 (right). Initial input (light line) and responding (bold line) cells were harvested and stained with different combinations of B220-APC, IgM^b-PE, and CD43-FITC. All panels were gated on B220⁺ cells. The presented histograms were obtained by overlaying individual histograms from three to four experiments, adding them together using CELLQuest™ software (Becton Dickinson) to obtain an average representative histogram, and normalizing them to the peak cell number. CTX, chemotaxis.