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. 2000 May 15;191(10):1699–1708. doi: 10.1084/jem.191.10.1699

Figure 2.

Figure 2

Figure 2

Figure 2

(A) Comparative analysis of DCs and cytokine-transduced tumor cells in preexisting pulmonary metastasis model. Animals were injected with 5 × 106 tumor cells by tail vein injection. DCs and cytokine-transduced tumor cell vaccines (5 × 105 cells) were given on days 3 and 7 by subcutaneous injection. All animals (n = 4/group) were killed on day 24 and the number of macroscopically visible metastases was determined. Error bars represent SE. (B) Comparative analysis of DCs and cytokine-transduced tumor cells in preexisting subcutaneous tumor model. Groups of 10 mice were treated with a single dose of Ad-transduced DCs (106) or irradiated cytokine-transduced tumor cells (both via subcutaneous administration) on day 5 after subcutaneous inoculation of 5 × 105 B16-MAGE-1 tumor cells. (C) MAGE-1 detection in transduced tumor cells and DCs. 10 μg of cell extract was loaded onto a 12% polyacrylamide gel and separated by electrophoresis. The 46-kD MAGE-1 protein was detected by the mAb MA454 (arrow).