Figure 5.

GM-CSF–transduced DCs give rise to higher cell counts in draining LNs. DCs were retrovirally transduced with CMMP-GFP or CMMP-GM-CSF-IRES-GFP and sorted for GFP⁺ expression. CMMP-GFP–transduced cells were additionally stained with the green CMFDA CellTracker™ dye; CMMP-GM-CSF-IRES-GFP⁺ cells were stained with the red fluorescent CMTMR CellTracker™ dye. Both populations were mixed immediately before subcutaneous injection. Animals were killed after 24 and 48 h and the ratio of green to red cells was determined in three draining LNs. The error bars indicate the SD of 10–20 sections/LN.