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. 2000 May 15;191(10):1807–1812. doi: 10.1084/jem.191.10.1807

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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48-kD sCD14 production by mammary epithelial cells and absence from commercial infant milk formulas and cows' milk. (A) Detection of sCD14 polypeptides by Western blotting in culture supernatants (Sup.) and total lysates (TL) from serum-free medium–cultured mammary epithelial cell line MCF-7. Parallel analysis of m-sCD14 in milk and normal human serum (NHS) is shown. Mr, relative molecular mass. (B) Infant milk formulas (IF, 1:6 and 1:25 dilutions), cows' milk (bottled milk [BM]), bovine colostrum (BC), and bovine plasma (BP) were tested for sCD14 by Western blotting. Shown is a representative result of 5 IF, 8 BM, 2 BC, and 10 BP.