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. 2000 May 15;191(10):1807–1812. doi: 10.1084/jem.191.10.1807

Figure 4.

Figure 4

IECs can be activated by LPS or whole bacteria by an m-sCD14–dependent mechanism. (A–D) IL-8, TNF-α, and ENA-78 production was tested by ELISA in supernatants of HT29 and SW620 cell lines cultured in medium supplemented with human milk (HM) and stimulated with E. coli LPS or varying numbers of whole E. coli (C and D; 2.5 × 106 E. coli). The anti-CD14 mAb MY4 (IgG2b) but not its isotype-matched control blocked LPS and bacterial stimulation. Representative results with HT29 cells are shown.