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. 2000 May 15;191(10):1777–1788. doi: 10.1084/jem.191.10.1777

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© 2000 The Rockefeller University Press

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Upregulation of CD80, CD86, and CD40 expression in DCs generated in the presence of type I IFN and GM-CSF. (A) Comparison with the effects induced by IL-4/GM-CSF at 3 and 6 d of culture. Freshly isolated monocytes were prepared as described in Materials and Methods. Cells were treated with GM-CSF (500 U/ml) and with 1,000 U/ml of either CIFN or IL-4. At 3 and 6 d, cell phenotype was evaluated by FACS^® analysis. Each bar is representative of four separate experiments. (B) Effects of different doses of type I IFN. Freshly isolated monocytes were isolated, cultured with cytokines, and analyzed for antigen expression on day 3, as described in Materials and Methods. Representative data from one out of three experiments are shown. (C) Effects of various preparations of type I IFN. Cultures were treated with 500 U/ml of GM-CSF and 1,000 U/ml of type I IFN, as indicated. FACS^® analysis was performed on day 3.