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. 2000 May 15;191(10):1777–1788. doi: 10.1084/jem.191.10.1777

Figure 7.

Figure 7

Potent in vivo primary antibody response to HIV-1 in hu-PBL-SCID mice immunized with autologous DCs generated in the presence of CIFN/GM-CSF and pulsed with inactivated HIV-1. (A) Human anti–HIV-1/gp41 antibodies (total Ig) in sera from hu-PBL-SCID mice immunized and boosted (7 d later) with 1.5 × 106 DCs pulsed (2 h at 37°C) with AT-2–inactivated HIV-1. Each bar represents the mean of values obtained in two distinct experiments (eight mice in total). CTR, sera from mice reconstituted with hu-PBLs and injected with DCs generated in the presence of IFN/GM-CSF, not pulsed with HIV-1. (B) Characterization of the Ig isotype of anti-gp41 antibodies in sera from immunized and control hu-PBL-SCID mice. (C) Western blot analysis of antibodies against HIV-1 proteins using pooled sera from immunized hu-PBL-SCID mice. Sera from three mice for each group were pooled. Results from two different experiments (Exp.) are shown. Western blot analysis was performed as described in Materials and Methods. CTR(−), sera from mice reconstituted with hu-PBLs and injected with DCs generated in the presence of IFN/GM-CSF, not pulsed with HIV-1; CTR(+), positive human serum.