Table 1.
Oligonucleotides and Restriction Enzymes for Chimera Construction
Chimera | Oligonucleotides | Restriction enzymes |
---|---|---|
A55/B | Sense: 5′-gggaagcttatggaaaccaacttct-3′ | HindIII, Eco47III |
Antisense: 5′-aacagcgcttcatccggaatccagc-3′ | ||
B/A62–262/B | Sense: 5′-gttagcgctcagtcaccaccatct-3′ | Eco47III, EcoNI |
Antisense: 5′-aacctcagccagggcaaccagttgaa-3′ | ||
B/A254 | Sense: 5′-gaacctggctgaggccgtctggctcaag-3′ | EcoNI, XhoI |
Antisense: 5′-cttctcgagtcacattgcctgtaac-3′ | ||
B/A299 | Sense: 5′-gtgcgccggcggctttgtgggccaagactt-3′ | SgrAI, XhoI |
Antisense: 5′-cttctcgagtcacattgcctgtaac-3′ |
See Materials and Methods for details.