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. 2000 Apr 3;191(7):1233–1240. doi: 10.1084/jem.191.7.1233

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© 2000 The Rockefeller University Press

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Target gene activation in TRAF6-deficient MEFs. (A) Failure of IL-17 to increase IL-6 production by the TRAF6-deficient EFs. Wild-type (+/+) or TRAF6-deficient (−/−) EFs were cultured in the presence of 10 ng/ml mTNF, 10 ng/ml IL-1, or 10 ng/ml mIL-17 for 15 h. IL-6 in cell culture medium was determined by ELISA. Data shown are average values of three independent experiments ± SD. (B) Failure of IL-17 to increase ICAM-1 expression on TRAF6-deficient cells. Wild-type or TRAF6^−/− cells were treated with cytokines as in A. Levels of ICAM-1 protein on cell surface were determined by FACS^® analysis using mouse mAbs specific to ICAM-1. Dotted line, untreated cells; solid line, cytokine-treated cells.

Target gene activation in TRAF6-deficient MEFs. (A) Failure of IL-17 to increase IL-6 production by the TRAF6-deficient EFs. Wild-type (+/+) or TRAF6-deficient (−/−) EFs were cultured in the presence of 10 ng/ml mTNF, 10 ng/ml IL-1, or 10 ng/ml mIL-17 for 15 h. IL-6 in cell culture medium was determined by ELISA. Data shown are average values of three independent experiments ± SD. (B) Failure of IL-17 to increase ICAM-1 expression on TRAF6-deficient cells. Wild-type or TRAF6^−/− cells were treated with cytokines as in A. Levels of ICAM-1 protein on cell surface were determined by FACS^® analysis using mouse mAbs specific to ICAM-1. Dotted line, untreated cells; solid line, cytokine-treated cells.