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. 2000 Apr 3;191(7):1177–1186. doi: 10.1084/jem.191.7.1177

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Isolation and expression of mouse Cats F and Z. (A) Northern blot analysis. Total RNA (20 μg/each) from mouse splenocytes, peritoneal macrophages, and flt-3–stimulated dendritic cells were separated on 1.2% agarose gel, blotted onto a nylon filter, and probed with full length mouse Cat F, Z, and S cDNAs. RNA loading control is shown by rRNAs (top panel). Both Cats F and Z can be detected from macrophages but not splenocytes or dendritic cells. In contrast, Cat S transcripts can be detected in all three cell types. (B) Amino acid sequence of mouse Cat Z. The active site amino acids are indicated with asterisks (*), and the potential sites for glycosylation are double underlined. Arrowheads indicate the potential cleavage sites of signal peptide and pro region of Cat Z. (C) Amino acid sequence of mouse Cat F. Arrowheads indicate the signal peptide and pro region cleavage sites. Three active site amino acids (Cys, His, and Asn) are indicated by asterisks (*), and three potential glycosylation sites are double underlined. The underlined region is the potential cystatin-like domain 32.

Isolation and expression of mouse Cats F and Z. (A) Northern blot analysis. Total RNA (20 μg/each) from mouse splenocytes, peritoneal macrophages, and flt-3–stimulated dendritic cells were separated on 1.2% agarose gel, blotted onto a nylon filter, and probed with full length mouse Cat F, Z, and S cDNAs. RNA loading control is shown by rRNAs (top panel). Both Cats F and Z can be detected from macrophages but not splenocytes or dendritic cells. In contrast, Cat S transcripts can be detected in all three cell types. (B) Amino acid sequence of mouse Cat Z. The active site amino acids are indicated with asterisks (*), and the potential sites for glycosylation are double underlined. Arrowheads indicate the potential cleavage sites of signal peptide and pro region of Cat Z. (C) Amino acid sequence of mouse Cat F. Arrowheads indicate the signal peptide and pro region cleavage sites. Three active site amino acids (Cys, His, and Asn) are indicated by asterisks (*), and three potential glycosylation sites are double underlined. The underlined region is the potential cystatin-like domain 32.