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. 2000 Apr 3;191(7):1137–1148. doi: 10.1084/jem.191.7.1137

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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CD28-dependent T cell absorption of various cell surface molecules from Dros. APCs. Preactivated CD8⁺ cells prepared from normal B6 (A, C, and D), CD28^−/− B6 (A and D), or ICAM-1^−/− B6 mice (B) were cultured with or without M17/4 anti–LFA-1 mAb (15 μg/ml) or an isotype-matched control (Cont.) mAb (anti-B220) with Dros. APCs expressing various mouse or human (h) cell surface molecules (L^d.ICAM-1 and L^d.B7-1.ICAM-1, A and B; HLA.hB7-1.hICAM-1, C; and IA^d.B7-1, for 1 h, D) and then stained for the markers indicated; staining of T cells before culture is shown as a control. (B) An anti-B220 mAb was used as a control. In other experiments, no inhibition was seen with mAbs specific for CD8, CD27, and TCR-β. The data show staining on gated CD8⁺ cells.

CD28-dependent T cell absorption of various cell surface molecules from Dros. APCs. Preactivated CD8⁺ cells prepared from normal B6 (A, C, and D), CD28^−/− B6 (A and D), or ICAM-1^−/− B6 mice (B) were cultured with or without M17/4 anti–LFA-1 mAb (15 μg/ml) or an isotype-matched control (Cont.) mAb (anti-B220) with Dros. APCs expressing various mouse or human (h) cell surface molecules (L^d.ICAM-1 and L^d.B7-1.ICAM-1, A and B; HLA.hB7-1.hICAM-1, C; and IA^d.B7-1, for 1 h, D) and then stained for the markers indicated; staining of T cells before culture is shown as a control. (B) An anti-B220 mAb was used as a control. In other experiments, no inhibition was seen with mAbs specific for CD8, CD27, and TCR-β. The data show staining on gated CD8⁺ cells.