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. 2000 Apr 3;191(7):1085–1094. doi: 10.1084/jem.191.7.1085

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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Induced Notch1^−/− BM generates splenic DCs in mixed BM chimeras. The BM chimeric mice described in Fig. 3 were also analyzed for splenic DC development. Spleens of chimeric mice were pooled, and the DC fraction was enriched as described in Materials and Methods. The DC-enriched fraction was stained using either anti-CD45.1 or anti-CD45.2, together with anti-F4/80, anti-CD11c, and anti-CD8α antibodies. The graph shows a FACS^® analysis of CD11c versus CD8α gated on F4/80⁻ cells for the two different groups of chimeric mice. Absolute numbers of the myeloid-related (CD11c⁺CD8α⁻) and lymphoid-related (CD11c⁺CD8α⁺) DCs per spleen are indicated next to the quadrants.