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. 2000 Apr 3;191(7):1127–1136. doi: 10.1084/jem.191.7.1127

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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DOαβGFP affects peptide presentation by HLA-DR3. (A) FACS^® analysis of 5,000 Mel JuSo cells transfected either with DOαβ² GFP (GFP⁺ population) or vector only (GFP⁻ population) showing staining with secondary antibody only (⁻), or staining that is specific for class II molecules (L243), CLIP-bound HLA-DR3 (CerCLIP.1), or HLA-DR3 complexed to stably bound peptides (16.23). (B) FACS^® analysis of 5,000 Mel JuSo cells expressing various levels of DOαβGFP, showing specific staining of HLA-DR3–CLIP complexes by CerCLIP.1 antibody. The vertical axis represents GFP and the horizontal axis PE-fluorescence derived from the secondary antibody, each in arbitrary units on a logarithmic scale. Values next to the right axis indicate which DOαβGFP populations were FACS^® sorted for analysis of relative DO/DM expression levels.

DOαβGFP affects peptide presentation by HLA-DR3. (A) FACS^® analysis of 5,000 Mel JuSo cells transfected either with DOαβ² GFP (GFP⁺ population) or vector only (GFP⁻ population) showing staining with secondary antibody only (⁻), or staining that is specific for class II molecules (L243), CLIP-bound HLA-DR3 (CerCLIP.1), or HLA-DR3 complexed to stably bound peptides (16.23). (B) FACS^® analysis of 5,000 Mel JuSo cells expressing various levels of DOαβGFP, showing specific staining of HLA-DR3–CLIP complexes by CerCLIP.1 antibody. The vertical axis represents GFP and the horizontal axis PE-fluorescence derived from the secondary antibody, each in arbitrary units on a logarithmic scale. Values next to the right axis indicate which DOαβGFP populations were FACS^® sorted for analysis of relative DO/DM expression levels.