Figure 3.

Elevated [K⁺]_o-induced T cell adhesion is blocked by Kv1.3 channel blockers and Sub P, and unaffected by nonrelevant blockers. ⁵¹Cr-labeled human T cells were incubated in elevated [K⁺]_o RPMI solutions and added with 10⁻⁹ to 10⁻⁷ M MgTx (A), KaTx (B), or NoTx (C), or with an additional series of relevant and nonrelevant ion channel blockers (D; as specified in the legend to Fig. 2D and Fig. E). The adhesion of the T cells to FN-coated wells was then measured. Similarly, human T cells were exposed either to CGRP (10⁻⁸ M; E) or to an elevated (25 mM) [K⁺]_o-containing solution (F), in the presence or absence of Sub P (10⁻⁸ M), and then tested for their adhesion to FN. BG, background adhesion. Each figure represents one of three experiments performed; data are expressed as the mean ± SD of four replicate microtiter wells.