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. 2000 Nov 20;192(10):1415–1424. doi: 10.1084/jem.192.10.1415

Figure 6.

Figure 6

The activation of coagulation factors bound to SR11B bacteria in the absence or presence of H-D-Pro-Phe-Arg-CMK. SR11B bacteria were preincubated with plasma in the absence or presence of H-D-Pro-Phe-Arg-CMK (50 μg/ml final concentration) for 15 min at room temperature. As a control, bacteria were incubated with buffer alone. Bacteria were washed as described in Materials and Methods and resuspended in a buffer containing the chromogenic substrate H-D-Pro-Phe-Arg-pNA·2HCl specific for F XII/PK (A) or H-D-Phe-Pip-Arg-pNA·2HCl specific for thrombin (B). After 30 min, bacteria were removed and the absorbance at 405 nm was determined.