Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Nov 20;192(10):1391–1402. doi: 10.1084/jem.192.10.1391

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

graphic file with name JEM001377.f5ab.jpg — Bcl-2 blocks grB-mediated Bax insertion and cytochrome c release but not Bid processing and translocation. (A) Vector-only transfected controls (neo) and Bcl-2–overexpressing Jurkats were treated with grB and Ad for the times indicated. After incubation, cells were fractionated and the proteins were resolved by SDS-PAGE on 15% gels and transferred to nitrocellulose. Cytosols were probed with a monoclonal anti–cytochrome c antibody. (B) The mitochondrial fraction from A was probed for Bid using a polyclonal anti-Bid antiserum. (C) Jurkat and Bcl-2–overexpressing Jurkat cells were treated with grB and Ad as indicated followed by subfractionation as described in Materials and Methods. The mitochondria were subjected to alkaline extraction followed by SDS-PAGE on 15% gels and transferred to nitrocellulose. Blots were probed with polyclonal anti-Bax antibodies. (D) Mitochondrial fractions from C were overexposed, scanned, and then analyzed with Image Gauge software to determine relative band intensities.

graphic file with name JEM001377.f5cd.jpg — Bcl-2 blocks grB-mediated Bax insertion and cytochrome c release but not Bid processing and translocation. (A) Vector-only transfected controls (neo) and Bcl-2–overexpressing Jurkats were treated with grB and Ad for the times indicated. After incubation, cells were fractionated and the proteins were resolved by SDS-PAGE on 15% gels and transferred to nitrocellulose. Cytosols were probed with a monoclonal anti–cytochrome c antibody. (B) The mitochondrial fraction from A was probed for Bid using a polyclonal anti-Bid antiserum. (C) Jurkat and Bcl-2–overexpressing Jurkat cells were treated with grB and Ad as indicated followed by subfractionation as described in Materials and Methods. The mitochondria were subjected to alkaline extraction followed by SDS-PAGE on 15% gels and transferred to nitrocellulose. Blots were probed with polyclonal anti-Bax antibodies. (D) Mitochondrial fractions from C were overexposed, scanned, and then analyzed with Image Gauge software to determine relative band intensities.