Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2000 Nov 20;192(10):1425–1440. doi: 10.1084/jem.192.10.1425

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

PMC Copyright notice

Typical protein immunogens have restricted distribution in cortices of draining lymph nodes after subcutaneous injection. Confocal micrographs of lymph node sections (10 μm) show FITC-labeled protein in subcapsular sinus (between arrows), highlighting reticular network and HEVs (arrowheads). Bars, 50 μm. (A) OVA-FITC (white) in the draining axillary lymph node. Lymph node was excised and fixed 15 min after injection of OVA into the mouse footpad. (B) HEL-FITC (green) in draining mouse lymph node. Lymph node was excised and fixed 5 min after injection of HEL into the footpad. Lymph node section was labeled topically with Cy-Chrome–labeled anti–mouse CD45R/B220 antibody to visualize B cells (red).