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. 2000 Nov 20;192(10):1425–1440. doi: 10.1084/jem.192.10.1425

Figure 5.

Figure 5

Lymph-borne molecules travel unidirectionally from the subcapsular sinus (arrows) through the reticular network toward HEVs (small arrowheads) in the lymph node cortex and are excluded from the lymphocyte microenvironments. Lymph node sections, 10 μm. Bars, 50 μm. (A) Lymphocytes, reticular fibers, and HEVs are visible in this confocal micrograph of a fresh-frozen section of mouse lymph node, topically labeled with FITC-labeled lectin, PSA (PSA-FITC). (B) Lymph-borne PSA-FITC highlights the subcapsular (arrows) and medullary (large arrowheads) sinuses and an incomplete reticular network starting at the subcapsular sinus. Small arrowheads point to three HEVs that are also highlighted by the lymph-borne PSA-FITC: two HEVs cut in cross-section (small arrowheads, middle right) and a single HEV with two branches, cut longitudinally (three small arrowheads, top left). Note the absence of labeled lymphocytes in the cortex. This draining lymph node was excised and fixed 30 min after injection of PSA-FITC into the footpad.