Table 1.
Summary of Tracer Characteristics and Their Distribution in Lymph Node Cortex
| Soluble molecule | MW | MR | SCS | IC | Fiber | HEV | Ref. |
|---|---|---|---|---|---|---|---|
| kD | nm | ||||||
| Acridine orange | 0.302 | 0.39 | + | + | 66 | ||
| Dextran 3 kD –Fluor or –TR | 3.0 | 1.01 | + | ± | + | + | 67 |
| LPS-FITC | 3.4–100 | 1.07–4.29 | + | − | − | − | 40, 66 |
| RANTES-Cy5 | 8.657 | 1.54 | + | − | + | + | 66, 68 |
| MIP-1α–FITC | 8.303 | 1.57 | + | − | + | + | 66, 68 |
| IL-8–FITC | 8.941 | 1.59 | + | − | + | + | 66, 68 |
| HEL | 14.3 | 1.93 | + | − | + | + | 39 |
| α-Lactalbumin (bovine) | 14.5 | 2.01 | + | − | + | + | 69 |
| Dextran 10 kD –Fluor or –TR | 10 | 2.22 | + | − | + | + | 70 |
| OVA | 48 | 2.73 | + | ± | + | + | 70 |
| HRP | 38 | 3.02 | + | ± | + | + | 71 |
| WGA | 43.2 | 3.04 | + | − | + | ± | 66 |
| PSA | 46 | 3.12 | + | − | + | ± | 66 |
| LCA | 50 | 3.23 | + | − | + | ± | 66 |
| BSA | 68 | 3.6 | + | − | + | + | 72 |
| Dextran 40 kD –Fluor or –TR | 37–40 | 4.0–5.0 | + | ± | + | + | 73 |
| LPS-Fluor | 3.4–100 | 1.07–4.29 | + | − | − | − | 40, 66 |
| IgG | 150 | 5.34 | + | − | − | − | 74 |
| Dextran 70 kD –Fluor or –TR | 70 | 5.5 | + | − | − | − | 75 |
| Dextran 500 kD –Fluor | 500 | 11.7 | + | − | − | − | 75 |
| Dextran 2,000 kD –TR | 2,000 | 16.0 | + | − | − | − | 75 |
SCS, subcapsular sinus; IC, intercellular, in the cortical parenchyma; Fiber, reticular fiber; Fluor, fluorescein; TR, Texas red. All other molecules labeled with FITC. Ref., reference(s).