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. 2000 Nov 20;192(10):1441–1452. doi: 10.1084/jem.192.10.1441

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© 2000 The Rockefeller University Press

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graphic file with name JEM000778.f3a.jpg — MIP-2 production by mast cells in vivo and in vitro. Microphotograph of an ear section stained for mast cells (A) with alcian blue and with anti–MIP-2 serum using immunoperoxidase staining (arrows). Controls using only primary or secondary Ab or preimmune serum showed no significant staining. MIP-2 mRNA expression of cultured mast cells (B) 24 h after stimulation with medium, ionomycin, or ionomycin and IL-1. Reverse transcription PCR was carried out with primers for MIP-2 mRNA (30 cycles). IL-1 alone did not induce MIP-2 mRNA expression. MIP-2 content in the supernatants from mast cells stimulated for 24 h with ionomycin and IL-1 was determined by ELISA (C). No or only very minor MIP-2 mRNA or MIP-2 protein was detected when mast cells were stimulated with either ionomycin or IL-1 alone.

graphic file with name JEM000778.f3b.jpg — MIP-2 production by mast cells in vivo and in vitro. Microphotograph of an ear section stained for mast cells (A) with alcian blue and with anti–MIP-2 serum using immunoperoxidase staining (arrows). Controls using only primary or secondary Ab or preimmune serum showed no significant staining. MIP-2 mRNA expression of cultured mast cells (B) 24 h after stimulation with medium, ionomycin, or ionomycin and IL-1. Reverse transcription PCR was carried out with primers for MIP-2 mRNA (30 cycles). IL-1 alone did not induce MIP-2 mRNA expression. MIP-2 content in the supernatants from mast cells stimulated for 24 h with ionomycin and IL-1 was determined by ELISA (C). No or only very minor MIP-2 mRNA or MIP-2 protein was detected when mast cells were stimulated with either ionomycin or IL-1 alone.