Table 1.

Proportion of T1, T2, Mature, and MZ B Cells in Freshly Prepared or Cultured BAFF-treated Surviving Splenocytes

	Fresh splenocytes(percentage oflive gate)	BAFF-treated splenocytes(percentage ofliving pool)	P values
Mature	36± 1.5	20± 0.1	<0.05
MZ	10± 1.3	2.2 ± 0.6	<0.01
T1	3.2 ± 0.4	0.7 ± 0.1	<0.01
T2	3.5 ± 0.3	53± 1.4	<0.01

Splenocytes were isolated from C57BL/6 mice and cultured with BAFF for 72 h. These cells as well as freshly prepared splenocytes from C57BL/6 mice were stained in parallel with a cocktail of anti-IgM, anti-IgD, and anti-CD21 antibodies and analyzed by flow cytometry. Various B cell subsets were gated according to their respective phenotype as in the legend to Fig. 3 B. The FSC versus SSC profile was obtained for each gated B cell subset and the percentage of surviving cells in the R2 gate is indicated for BAFF-treated splenocytes. For fresh control splenocytes, percentage within the live gate is indicated.