Table 2.
Proportion of T1, T2, Mature, and MZ B Cells in the Spleen of Control Versus BAFF Tg Mice
| T1 | T2 | Mature | MZ | |
|---|---|---|---|---|
| Percentage from gated cells | ||||
| Control | 13 ± 1.8 | 23 ± 7.6 | 67 ± 9.2 | 7.8 ± 1.75 |
| BAFF Tg | 20 ± 7 | 57 ± 13 | 29 ± 11.6 | 17 ± 2.8 |
| P values | >0.05 | <0.01 | <0.01 | <0.01 |
| Absolute numbers (×106) | ||||
| Control | 2.1 | 5 | 17 | 1.6 |
| BAFF Tg | 24 | 75 | 55 | 54 |
| P values | <0.01 | <0.01 | <0.05 | <0.01 |
Splenocytes isolated from control littermates and BAFF Tg mice were stained in parallel with a cocktail of anti-IgM, anti-IgD, and anti-CD21 antibodies and analyzed by flow cytometry. Various B cell subsets were gated according to their respective phenotype as in the legend to Fig. 3 B. Percentage from gated CD23+ cells is shown for T2 and mature B cells and from gated CD23− cells for T1 and MZ B cells. Corresponding absolute numbers per spleen are also shown. Results are representative of at least 10 animals analyzed per group. P values were obtained using ANOVA.