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Expression of Bcl-XL in naive T cells does not restore normal cytokine production in DO.11 CD28−/− T cells. Naive spleen and lymph node CD4+ lymphocytes from the retrovirus-infected stem cell–reconstituted mice described in the legend to Fig. 5 were activated for 4 d in vitro with 1 μg/ml of OVA peptide and APCs and analyzed for IL-4 production by intracellular cytokine staining. FSC, forward scatter.
