Table 2.
Representation of CD4+ and CD8+ T Cell Clones in Perivascular and Parenchymal Locations of the Four Lesions Analyzed for Case 1
| Single parenchymal CD8+ T cells | Single parenchymal CD4+ T cells | Samples of perivascular cells | ||||
|---|---|---|---|---|---|---|
| Lesion | Clone no. | Frequency | Clone no. | Frequency | Clone no. | Frequency |
| No. 1 | 1 | 14/29 (48%) | 1 | 8/41 (20%) | ||
| 9 | 3/29 | 6 | 2/41 | |||
| 3, 4, 7, 15, 21 | 1/29 | 12 | 1/3 | 3, 4, 13,17 | 1/41 | |
| Sum: | 22/29 (76%) | Sum: | 14/41 (34%) | |||
| No. 2 | 1 | 6/28 (21%) | ||||
| 2 | 3/28 | 1, 8 | 3/25 | |||
| 9 | 2/28 | 2 | 2/25 | |||
| 3, 16, 18, 19 | 1/28 | 11 | 1/2 | 7, 10 16, 21 | 1/25 | |
| Sum: | 15/28 (54%) | Sum: | 12/25 (48%) | |||
| No. 3 | 1 | 8/24 (33%) | 1 | 4/14 | ||
| 16, 18 | 2/24 | 10, 11 | 2/19 | 4 | 2/14 | |
| 4, 14, 15, 17 | 1/24 | 12, 13 | 1/19 | 9, 14 | 1/14 | |
| Sum: | 16/24 (67%) | Sum: | 6/19 (32%) | Sum: | 8/14 | |
| No. 4 | 1 | 3/6 | ||||
| 10 | 1/13 | 9, 15 | 1/6 | |||
| Sum: | 5/6 | |||||
| Sum | 1 | 28/81 (35%) | ||||
| 9 | 5/81 | 1 | 18/86 (21%) | |||
| 2, 16, 18 | 3/81 | 10, 11 | 3/37 | 4, 8 | 3/86 | |
| 3, 4, 15 | 2/81 | 12 | 2/37 | 2, 6, 9 | 2/86 | |
| 7, 14, 17, 19, 21 | 1/81 | 13 | 1/37 | 3, 7, 10, 13, 14, 15, 16, 17, 21 | 1/86 | |
| Sum: | 53/81 (65%) | Sum: | 9/37 (24%) | Sum: | 39/86 (45%) | |
Clonal expansions were identified by amplification of an identical rearrangement from at least two different samples of cells micromanipulated from material of case 1, and were numbered arbitrarily. Except for clones 6 and 8, all clones could be assigned to the CD8+ or CD4+ subset, as the clonal V region sequence was obtained at least once from a single parenchymal CD8+ or CD4+ cell. Only potentially functional rearrangements are listed. Three clones defined by nonfunctional rearrangments accounted for 7 of the 24 nonfunctional rearrangments obtained in total. These clonal sequences could not be assigned to clones defined by potentially functional rearrangements. The population of parenchymal T cells was dominated by CD8+ T cells (CD8/CD4 ≈ 3, as determined in lesions 3 and 4). Likewise, perivascular CD8+ T cells outnumbered perivascular CD4+ T cells roughly by a factor of three.