Table 3.

Representation of CD4⁺ and CD8⁺ T Cell Clones in Perivascular and Parenchymal Locations of the Single Lesion Analyzed for Case 2

Single parenchymal CD8+ T cells		Single parenchymal CD4+ T cells		Samples of perivascular cells
Clone no.	Frequency	Clone no.	Frequency	Clone no.	Frequency
2	4/24 (17%)
5	3/24			8	3/46 (7%)
3, 6, 9	2/24			1, 2, 7	2/46
4, 8, 7, 10	1/24	13, 14, 15	2/52	3, 4, 10	1/46
Sum:	17/24 (71%)	Sum:	6/52 (12%)	Sum:	12/46 (26%)

Clonal expansions were identified by amplification of an identical rearrangement from at least two different samples of cells micromanipulated from material of case 2, and were numbered arbitrarily. Except for clone 1, all clones could be assigned to the CD8⁺ or CD4⁺ subset, as the clonal V region sequence was obtained at least once from a single parenchymal CD8⁺ or CD4⁺ cell. Only potentially functional rearrangments are listed. One clone defined by a nonfunctional rearrangement accounted for 2 of the 17 nonfunctional rearrangements obtained in total. This clone could not be assigned to a clone defined by a potentially functional rearrangement. Within the parenchyma, CD8⁺ T cells outnumbered CD4⁺ T cells by a factor of five to six, and roughly by a factor of three in the perivascular location.