Table 4.

Case 1: CDR3 Nucleotide and Deduced Amino Acid Sequences of TCR-β Gene Rearrangements Amplified from Micromanipulated T Cells

Clone/sample	Vβ		VNDN	J	Jβ	CDR3 length
A278	11S1	CASSESGSG		EKLFFG	1S4	10
		TGTGCCAGCAGTGAATCCGGATCTGGC		GAAAAACTGTTTTTTGGC
7	11S1		CASSESGSG	EKLFFG	1S4	10
		TGTGCCAGCAGTGAAAGTGGGTCGGGG		GAAAAACTGTTTTTTGGC
1	6S5	CASSLSGQG		DYGYTF G	1S2	11
H518	13S3	CATGVS GSGG		EQFFG	2S1	10

Sequences A278 and H518 were both amplified from samples of perivascular cells (CD8⁺ clones 1 and 7 are introduced in Table ). CDR3 sequences are shown from the conserved cysteine of the Vβ to the conserved FG motif of the Jβ elements. Amino acids of the common SGSG motif are shown in bold. CDR3 lengths definition was as in Moss and Bell (reference 66).