Table 3.

Cytokine Production after In Vivo Transfer of IRF-2^+/− and IRF-2^−/− CD4⁺ T Cells into RAG-1^−/− Mice

	Transferred cells
	–		IRF-2+/+ B cells		IRF-2+/+ B cells +IRF-2+/− CD4+		IRF-2+/+ B cells +IRF-2−/− CD4+
Parasite burden	3.0 × 107 ± 2.7 × 107		7.2 × 106 ± 3.6 × 106		8.7 × 104 ± 1.3 × 104		4.0 × 104 ± 1.3 × 104
	In vitro stimulus
	−LmAg	+LmAg	−LmAg	+LmAg	−LmAg	+LmAg	−LmAg	+LmAg
IFN-γ (pg/ml)	<40	<40	<40	<40	80 ± 0	3,500 ± 70	<40	2,000 ± 0
IL-4 (pg/ml)	<5	<5	<5	<5	55 ± 35	375 ± 250	<5	50 ± 14

6 wk after the start of the experiment, splenocytes were prepared from L. major–infected RAG-1^−/− mice (three mice per group) as described in Materials and Methods. Parasite burden (expressed as the number of leishmania per spleen) and cytokine production in pooled cells from each group were determined as described in Materials and Methods. Data are shown as mean ± SD of triplicate determinations.