Table 4.

In Vitro Differentiation of CD4⁺ IRF-2^+/− and IRF-2^−/− T Cells

	IRF-2+/−				IRF-2−/−
	IFN-γ		IL-4		IFN-γ		IL-4
Secondary stimulus:	−	anti-CD3	−	anti-CD3	−	anti-CD3	−	anti-CD3
Primary stimulus
Anti-CD3	<0.016	6	<0.0075	<0.0075	<0.016	<0.016	<0.0075	<0.0075
Anti-CD3 + IL-4 + anti–IL-12	<0.016	13	<0.0075	65	<0.016	50	<0.0075	63
Anti-CD3 + IL-12 + anti–IL-4	1	1,260	<0.0075	<0.0075	<0.016	600	<0.0075	<0.0075

Pooled naive CD4⁺ T cells were purified from spleens and LNs of IRF-2^−/− and control IRF-2^+/− mice. The cells were stimulated with immobilized anti-CD3 and the indicated (primary stimulus) cytokines and antibody for 96 h, then washed and transferred to wells without anti-CD3. After an additional 48 h, the cells were washed and restimulated in the presence or absence of anti-CD3 (secondary stimulus). SNs were harvested after 24 h and analyzed in triplicate for IFN-γ and IL-4 production (in ng/ml) by ELISA. In all cases, the SD of these determinations was <10%.