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. 2000 Aug 21;192(4):537–548. doi: 10.1084/jem.192.4.537

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© 2000 The Rockefeller University Press

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/4.0/).

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TCRαβDN cells do not require MHC-dependent positive selection for development. Thymocytes were isolated from MHC class I–specific (HY and P14) or class II–specific (AND, 5CC7, and DO11.10) TCR transgenic mice bred onto a positively selecting (pos) or nonselecting, neutral (neut) MHC background. Cells were stained for CD4, CD8, and TCR (using antibodies: T370 for HY, anti-Vα2 for P14, anti-Vα11 for AND and 5CC7, and KJ-126 for DO11.10 TCR). The percent of transgenic (tg) TCR^hi cells was determined from analysis of total thymocytes by software gating for CD4⁻CD8⁻, CD4⁻8⁺, or CD4⁺CD8⁻. Each bar represents the mean percentage (with SE bars) of TCR^hi of CD4⁻CD8⁻ or of total thymocytes from analyses of three to six individual mice per group.