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CD4−CD8− T cells of AND and HY TCR mice express transgenic TCRαβ but no TCRγδ, while those of P14 TCR mice coexpress transgenic TCRαβ and endogenous TCRγδ. Thymocytes and lymph node cells, stained for TCRαβ (H57-597), TCRγδ (GL3), CD4, and CD8, were analyzed by flow cytometry using live gating to collect data only from CD4−CD8− cells. The numbers inside the quadrants represent the percentage of CD4−CD8− thymocytes in each population. Statistics are given in Table .
